biocommaIntroduction
Mycotoxins are toxic secondary metabolites produced byfungi, primarily absorbed by humans through diet. Aspergillus,Penicillium, and Fusarium are the main genera generating themost common mycotoxins found in food, including aflatoxin,ochratoxin A, sterigmatocystin, patulin, fumonisin, zearalenone,deoxynivalenol, NIV, T-2 toxin, etc. Biocomma establisheda high-throughput LC-MS/MS method using Copure® 302Multifunctional Purification Plate for detection of 10 mycotoxinsin corn flour with good recovery and stability for your reference.
Experiment
Extraction
Weigh 5 g of sample into a 50 mL centrifuge tube. Add 20 mLof acetonitrile-water-acetic acid solution (80:19:1) and vortexto mix well. Then, extract by ultrasonic treatment for 20 min.Centrifuge at 6000 r/min for 10 min. The supernatant is readyfor purification.
Purification (Copure® 302 Multifunctional PurificationPlate)
Place a 24 well purification plate on a 24 well collectionplate. Add 6 mL of supernatant to the purification plate. Placethe 24 well purification plate and the collection plate on theBiocomma® Positive Pressure 24 Processor. Turn on thegas. Make sure the wells of purification plate are positioneddirectly under the gas vent. Adjust the gas flow until all thesample filter into the collection plate. Pipette 4 mL of sampleto a clean tube. Add 20 μL of acetic acid. Evaporate sampleto dryness at 40 ℃ . Re-dilute to 1 mL by acetonitrile-watersolution (50:50). Vortex for 30 seconds to dissolve the residue.After filter by 0.22 μm microporous membrane, the sample isready for analysis.
Instrument Conditions
1.Chromatography Conditions
Equipment: UPLC-MS/MS (Thermo Scientific TSQ Endura)
Chromatographic column: Commasil® BEH T-C18 (2.1mm×100 mm,3 μm)
Mobile phase: A:5mM Ammonium acetate solution (contains0.1% formic acid)
Mobile phase: B: Acetonitrile (contains 0.1% formic acid)
Mobile phase gradient: initial 90%A,40%A (0 min~2 min), 10%A(2 min~6 min), 10%A (6min~7 min), 90%A (7 min~8 min),90%A (8 min~10 min)
Flow rate: 0.3 ml/min
Column temperature: room temperature
Injection volume: 5.0 μL
2.Mass Spectrometry Conditions
Auxiliary gas pressure: 8 arb
Sheath gas pressure: 30 arb
Ion exchange tube: 300 ℃
Auxiliary air temperature: 350 ℃
Detection method: MRM
Scan method: positive ion mode (ESI+) and negative ion mode(ESI-)
Note: The detection of zearalenone is in negative ion mode(ESI-), and the others are in positive ion mode (ESI+).
Results
Table 2. Spiked Recovery of 10 Mycotoxins in Corn Flour
Based on Table 2, the recovery rates of 10 Mycotoxins areall between 90-110% with RSD less than 10%, which meetthe standard of the experimental requirements.
Ordering Information
