biocommaIntroduction
Patulin is commonly found in fruits, fruit products and fruit wine.Patulin is a strong toxin to humans and animals, potentiallyleading to respiratory issues.
Biocomma established a high-throughput LC method usingCopure® 228 Multifunctional Purification Plate for detectionof patulin in apple cider with good recovery and stability foryour reference. The recoveries were 90-110%, and CV valuebetween wells was less than 5% at both 5 ng/g and 10 ng/g concentrations. This method is also applicable to fruit andvegetable juices and alcoholic foods.
Experiment
Extraction
Weigh 4 g of sample and dilute to 25 mL with acetonitrile.Vortex for 5 min and centrifuge at 6000 r/min for 5 min. Thesupernatant is ready for purification.
Purification (Copure® 228 Multifunctional PurificationPlate)
Place a 24 well purification plate on a 24 well collectionplate. Add 6 mL of supernatant to the purification plate. Placethe 24 well purification plate and the collection plate on theBiocomma® Positive Pressure 24 Processor. Turn on thegas. Make sure the wells of purification plate are positioneddirectly under the gas vent. Adjust the gas flow until all thesample filter into the collection plate. Pipette 4 mL of sampleto a clean tube. Add 20 μL of acetic acid. Evaporate sample todryness at 40 ℃ and redissolve by 1 mL of initial mobile phase.Vortex for 30 seconds to dissolve the residue. After filter by 0.22μm microporous membrane, the sample is ready for analysis.
Instrument Conditions
Equipment: UltiMate 3000 (Thermo Fisher Scientific) with DADdetector.
Chromatographic column: Agilent ZORBAX C18 (4.6 mm×250mm, 5 μm)
Mobile phase: A: Deionized water, B: Acetonitrile
Mobile phase gradient: 5% B (0min~13min), 100% B(13min~15min), 5% B (15min~20min)
Flow rate: 1.0 mL/min
Column temperature: 40 ℃
Injection volume: 20.0 μL
Detection wavelength: 276 nm
Detector: DADResults
Based on Figure 1, less miscellaneous peaks showingthe impurities are obviously absorbed by Copure® 229Multifunctional Purification Plate. There are no interferencepeaks next to the target peak, so the quantification is moreaccurate.
Table 1. Patulin Spike Recovery in Apple Cider
Based on Table 1, the recovery rates of patulin in the 24 wellsare all between 90-110%, and the CV value of the recoveryrate between wells is less than 5%, which meet the standard ofthe experimental requirements.
Ordering Information
