biocommaIntroduction
Zearalenone, a non-steroidal mycotoxin from Fusarium fungus,is commonly found in contaminated grains (wheat, corn, oats,barley, etc.). It’s a major global crop contaminant, leadingto adverse effects such as tumors, estrogen presence, andimmune suppression in livestock and poultry that consumecontaminated feed, and then poses a risk to human health.
Biocomma established a high-throughput LC method usingCopure® 224 Multifunctional Purification Plate for detection ofzearalenone in corn flour with good recovery and stability foryour reference. The recoveries were 90-110%, and CV valuebetween wells was less than 5% at both 8 ng/g and 16 ng/gconcentrations.
Experiment
Extraction
Weigh 5 g of sample into a 50 mL centrifuge tube. Add 1 g ofsodium chloride and 20 mL of acetonitrile-water solution (9+1).Vortex for 15 min. Centrifuge at 6000 r/min for 5 min. Thesupernatant is ready for purification.
Purification (Copure® 224 Multifunctional PurificationPlate)
Place a 24 well purification plate on a 24 well collection plate.Add 6 mL of supernatant to the purification plate. Place the 24well purification plate and the collection plate on the Biocomma®Positive Pressure 24 Processor. Turn on the gas. Make surethe wells of purification plate are positioned directly underthe gas vent. Adjust the gas flow until all the sample filter intothe collection plate. Pipette 4 mL of sample to a clean tube.Evaporate sample to dryness at 40 ℃ and redissolve by 1 mLof initial mobile phase. Vortex for 30 seconds to dissolve theresidue. After filter by 0.22 μm microporous membrane, thesample is ready for analysis.
Instrument Conditions
Equipment: UltiMate 3000 (Thermo Fisher Scientific) with FLDdetector
Chromatographic column: Agilent ZORBAX C18 (4.6 mm×250mm, 5 μm)
Mobile phase: A: Acetonitrile, B: Deionized water, C: MethanolFlow rate: 1.0 mL/min
Column temperature: room temperature
Injection volume: 50.0 μL
Detection wavelength: excitation wavelength 274 nm, emissionwavelength 440 nm
Detector: FLD
Table 1. Gradient Elution Program
Results
Based on Figure 1, the pigments are obviously absorbed byCopure® 226 26 Multifunctional Purification Plate.
Table 2. Zearalenone Spike Recovery in Corn Flour
Based on Figure 2 and Table 2, the miscellaneous peaks of purified sample are much fewer indicating obviously absorbedimpurities. The recovery rates of aflatoxin in the 24 wells are between 90-110%, and the CV value is less than 5%, which meetthe standard of experimental requirements.
Ordering Information
